Partek Flow Documentation

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From the GSA the DESeq2 task report, we can browse to any gene in the Chromosome Viewview.

  • Select Click  in the SELM SELENOM row to open Chromosome View view (Figure 1)

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SubtitleTextBrowsing to a location in Chromosome View
AnchorNameBrowsing to location

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A new tab will open showing SELM SELENOM in the Chromosome View view (Figure 2).


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SubtitleTextViewing SELM in Chromosome view
AnchorNameSELM in Chromosome view

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Chromosome View shows reference genome, annotation, and data set information together aligned at genomic coordinates. 

Each track has Configure track  and Move track  buttons that can be used to modify each track. 

The top track (Figure prev.) shows average number of total count normalized reads for each of the three treatment groups in a stacked histogram. The second track shows the RefSeq annotation.

We can add tracks from any data node using Select Tracks.

 

  • Click Select Select tracks

A pop-up dialog showing the pipeline allows us to choose which data to display as tracks in Chromosome Viewview (Figure 3).

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SubtitleTextChoosing tracks to display in Chromosome view
AnchorNameChoosing tracks to display

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  • Click Reads pileup under Align Aligned reads on the left-hand side of the dialog 
  • Select Click Display tracks to make the change

The reads pileup track is now included (Figure 4).

 

Selecting a read brings up detailed information about the read in the selection details panel (Figure).

 


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SubtitleTextThe reads pileup track shows every read in its aligned position on the reference genome
AnchorNameReads pileup track

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To learn more about Chromosome Viewviewplease consult the Chromosome View user guide.

 


 

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