Page History
The significant CpG loci detected in the previous step actually form a methylation signature , which we shall now visualise by that differentiates between LCLs and B cells. We can build and visualize this methylation signature using clustering and a heat map. Start by selecting the shPOU5F1_vsPrimed
- Select the LCLs_vs_Bcells_CpG_Islands spreadsheet in the spreadsheet pane on the left
...
- Select Cluster Based on Significant Genes from the
...
Section Heading
Section headings should use level 2 heading, while the content of the section should use paragraph (which is the default). You can choose the style in the first dropdown in toolbar.
- Visualization panel of the Illumina BeadArray Methylation workflow
- Select Hierarchical Clustering for Specify Method (Figure 1)
Numbered figure captions | ||||
---|---|---|---|---|
| ||||
- Select OK
- Verify that LCLs_vs_Bcells_CpG_Islands is selected in the drop-down menu
- Verify that Standardize is selected for Expression normalization (Figure 2)
Numbered figure captions | ||||
---|---|---|---|---|
| ||||
- Select OK
The heat map will be displayed on the Hierarchical Clustering tab (Figure 3).
Numbered figure captions | ||||
---|---|---|---|---|
| ||||
Page Turner | ||
---|---|---|
|
Additional assistance |
---|
|
Rate Macro | ||
---|---|---|
|
Overview
Content Tools