Partek Flow Documentation

Page tree

Versions Compared

Key

  • This line was added.
  • This line was removed.
  • Formatting was changed.

...

By default, all samples are used to perform QA/QC. If there is more than one sample, you You can choose to split the sample and perform QA/QC separately for each sample. 

...

Numbered figure captions
SubtitleTextSelect & Filter criteria
AnchorNameSelect & Filter Single cell QAQC

Image Modified

Hovering the mouse over one of the selection criteria reveals a histogram showing you the frequency distribution of the respective quality metric. The minimum and maximum thresholds can be adjusted by clicking and dragging the sliders or by typing directly into the text boxes for each selection criteria (Figure 6).

...

Numbered figure captions
SubtitleTextUse the sliders and histograms to adjust the selection criteria
AnchorNameSelecting cells using sliders

Image Modified

Alternatively, Pin histogram to view all of the distributions at one time to determine thresholds with ease (Figure 7).

...

Numbered figure captions
SubtitleTextPin the histogram to view all of the distributions at one time
AnchorNamePin histogram

Image RemovedImage Added

Adjusting the selection criteria will select and deselect cells in all three plots simultaneously. Depending on your settings, the deselected points will either be dimmed or gray. The filters are additive. Combining multiple filters will include the intersection of the three filters. The number of cells selected is shown in the figure legend of each plot (Figure 8).

...

Numbered figure captions
SubtitleTextThe filters are additive and deselected cells are dimmed
AnchorNameSingle cell QAQC with selection criteria applied

Image RemovedImage Added

To filter the high-quality cells, click the include selected cells  icon in Filter in the top right of Select & Filter, and click Apply observation filter... (Figure 9).

...

Numbered figure captions
SubtitleTextAfter the Apply filter button is selected, you will be presented with a preview of your pipeline. You need to select the appropriate data node to apply the filtering to
AnchorNameSelect input data node for filtering

Image Modified

A new data node, Filtered counts, will be generated under the Analyses tab (Figure 11).

...

Numbered figure captions
SubtitleTextFiltered counts task report
AnchorNameFilter counts report

Image RemovedImage Added




Additional assistance


...