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By default, Trim bases removes bases removes bases starting at the 3' end and continuing until it finds a base pair call with a Phred score of equal to or greater than 20. Hover over the for additional information on any task option 35 (Figure 2).
- Click Finish to run Trim bases with default settings
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The results are fairly consistent across samples with ~50% ~2% of reads untrimmed, ~45% ~86% trimmed, and ~5% ~12% removed for each. The average quality score for each sample is increased with higher average quality scores at the 3' ends.
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