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- Select Add Sample Attributes in the Import section of the Gene Expression workflow panel
- Choose the option Add Attributes from an Existing Column and select
- Select OK to open the Sample Information Creation dialog
In In this tutorial, the file name (e.g., Down Syndrome-Astrocyte-748-Male-1-U133A.CEL) contains the information about a sample and is separated by hyphens (-). Choosing to split the file name by delimiters will separate the categories into different columns
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- Select the Random Effect check box from the Properties dialog (Figure 3) then select OK.
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The column 5. (Subject) will now be colored red, indicating that it is a random effect.
- To save changes to the spreadsheet, select the Save Active Spreadsheet icon ( ). Spreadsheets with unsaved changes have an asterisk next to their name in the spreadsheet tree.
Note: More details on Random vs. Fixed Effects can be found later in this tutorial under the section Identifying Differentially Expressed Genes differentially expressed genes using ANOVA.
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