Partek Flow Documentation

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SubtitleTextInvoking the Trim bases task
AnchorNameTrim bases

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By default, Trim bases removes bases removes bases starting at the 3' end and continuing until it finds a base pair call with a Phred score of equal to or greater than 20. Hover over the Image Removed for additional information on any task option 35 (Figure 2). 

  • Click Finish to run Trim bases with default settings

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SubtitleTextViewing additional information about the Quality score trimming methodConfiguring Trim bases
AnchorNameConfiguring Trim bases

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The Trim bases task will generate a new data node, Trimmed reads (Figure 3). We can view the task report for Trim bases by double-clicking either the Trim bases task node or the Trimmed reads data node or choosing Task report from the task menu. 

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SubtitleTextA Task and a Data node are created from the Trim bases task. Task and Data nodes that have been queued or are in progress are shown in a lighter color than completed tasks.
AnchorNameQueued task node

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  • Double-click the Trimmed reads data node to open the task report

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SubtitleTextResults of the Trim bases task
AnchorNameTrim bases report

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The results are fairly consistent across samples with ~50% ~2% of reads untrimmed, ~45% ~86% trimmed, and ~5% ~12% removed for each. The average quality score for each sample is increased with higher average quality scores at the 3' ends. 

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