Page History
The significant CpG loci detected in the previous step actually form a methylation signature that differentiates , which we shall now visualise by between LCLs and B cells. We can build and visualize this methylation signature using clustering and a heat map.
- Select the LCLs vs. B cells _vs_Bcells_CpG_Islands spreadsheet in the spreadsheet pane on the left
- Select Cluster Based on Significant Genes from the Visualization panel of the Illumina BeadArray Methylation workflow
- Select Hierarchical Clustering for Specify Method (Figure 1)
Numbered figure captions | ||||
---|---|---|---|---|
| ||||
- Select OK
- Verify that LCLs vs. B cells is _vs_Bcells_CpG_Islands is selected in the drop-down menu
- Select Verify that Standardize is selected for Expression normalization (Figure 2)
Numbered figure captions | ||||
---|---|---|---|---|
| ||||
- Select OK
The heat map will be displayed on the Hierarchical Clustering tab (Figure 3).
Numbered figure captions | ||||
---|---|---|---|---|
| ||||
...
Overview
Content Tools