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The tutorial data set includes 9 samples equally divided into 3 treatment groups. Sequencing was performed by an Illumina HiSeq (paired-end reads), but the workflow can be easily adapted for data generated by other sequencers. Each sample has 2 fastq files for a total of 18 fastq files with the combined size of 6.17 GB.
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You can obtain the tutorial data set through Partek Flow.
- Click your avatar
- Click Settings in the drop-down menu (Figure 1)
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At the top of the System information page, there is a section labeled Download tutorial data (Figure 2).
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- Click RNA-Seq 5-AZA to download the tutorial data set
A new project will be created and you will be directed to the Analyses Tab. The data will be downloaded automatically (Figure 3) and imported into your project. Because this is a tutorial project, there is no need to click on Add data as it will be done automatically.
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At first the project is empty, but the file download will start automatically in the background. You can wait a few minutes then refresh your browser or you can monitor the download progress using the Queue.
- Click Queue
- Click View Queued Tasks in the drop-down menu
The Queued tasks page will open (Figure 4).
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- Click Projects
- Click RNA-Seq 5-AZA in the drop-down menu
The Analyses tab will open (Figure 5). If you download has completed, you will see a blue circle titled mRNA.
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Once the download completes, the sample table will appear in the Metadata tab.
- Click the Metadata tab
The Metadata tab includes the sample table with the names of each imported sample (Figure 6).
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In the next section of the tutorial, we will add a sample attribute that indicates the treatment group of each sample.
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