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The significant CpG loci detected in the previous step actually form a methylation signature that differentiates between LCLs and B cells. We can build and visualize this methylation signature using clustering and a heat map.
- Select the LCLs_vs_Bcells_CpG_Islands spreadsheet in the spreadsheet pane on the left
- Select Cluster Based on Significant Genes from the Visualization panel of the Illumina BeadArray Methylation workflow
- Select Hierarchical Clustering for Specify Method (Figure 1)
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- Select OK
- Verify that LCLs_vs_Bcells_CpG_Islands is selected in the drop-down menu
- Verify that Standardize is selected for Expression normalization (Figure 2)
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- Select OK
The heat map will be displayed on the Hierarchical Clustering tab (Figure 3).
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