Partek Flow Documentation

Page tree

Versions Compared

Key

  • This line was added.
  • This line was removed.
  • Formatting was changed.
Comment: Reverted from v. 7

Table of Contents
maxLevel2
minLevel2
excludeAdditional Assistance

From the GSA task report, we can browse to any gene in the Chromosome view.

  • Click  in the SELENOM row to open Chromosome view (Figure 1)

Numbered figure captions
SubtitleTextBrowsing to a location in Chromosome View
AnchorNameBrowsing to location

 

A new tab will open showing SELENOM in the Chromosome view (Figure 2).


Numbered figure captions
SubtitleTextViewing SELM in Chromosome view
AnchorNameSELM in Chromosome view

Chromosome View shows reference genome, annotation, and data set information together aligned at genomic coordinates. 

Each track has Configure track  and Move track  buttons that can be used to modify each track. 

The top track shows average number of total count normalized reads for each of the three treatment groups in a stacked histogram. The second track shows the RefSeq annotation.

We can add tracks from any data node using Select Tracks.

  • Click Select tracks

A pop-up dialog showing the pipeline allows us to choose which data to display as tracks in Chromosome view (Figure 3).


Numbered figure captions
SubtitleTextChoosing tracks to display in Chromosome view
AnchorNameChoosing tracks to display

  • Click Reads pileup under Aligned reads on the left-hand side of the dialog 
  • Click Display tracks to make the change

The reads pileup track is now included (Figure 4).


Numbered figure captions
SubtitleTextThe reads pileup track shows every read in its aligned position on the reference genome
AnchorNameReads pileup track

With multiple tracks, it may be useful to pin a track to the top so we can scroll down the reads pileup track without losing sight of the Alignments or RefSeq tracks. 

  • Click  next to the Alignments track
  • Click  to pin the track to the top 
  • Repeat for RefSeq Transcripts 
  • Scroll down to view individual reads 

Selecting a read brings up detailed information about the read in the selection details panel (Figure 5).


Numbered figure captions
SubtitleTextViewing read information in the Reads pileup track
AnchorNameViewing read information

To learn more about Chromosome view, please consult the Chromosome View user guide.


Page Turner
button-linkstrue


Additional assistance


Rate Macro
allowUsersfalse