Introduction

Partek ^® Flow^®   has a List generator function that allows you to investigate unique and common features between different lists within a project. These can be visualized in a Venn diagram consisting of up to five sets. 

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Feature lists can be generated by RNA-Seq tasks such as GSA and ANOVA. Filter your feature lists as needed. In the example below, three feature lists were generated by filtering the same multivariate GSA result on three different contrasts. Each filtered list appears as its own layer on the Analyses tab. 

Selecting Lists for Venn Diagram

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Note that you must be a collaborator in the project to see the purple List Generator button. 

If the current project does not contain any feature lists, a message will appear.

The List name field can be customized to provide specific information about the selected list and will also serve as the set's name on the Venn diagram. Hovering your mouse over a specific feature list highlights its node location on the task graph.  

 Conversely, clicking a feature list data node highlights the feature list on the right panel. 

 Click on the  icon to select the identifier that will be used to compare between the sets. The Select identifier dialog box will then appear.

For your convenience, example entries for each possible identifier is displayed. Select the radio button of the identifier you would like to use for comparison. Note that the List generator only uses Identifiers to determine common features within a list. For feature lists generated by automated tasks like Cufflinks' novel transcript discovery function, keep in mind that these identifiers may not be referring to the same feature. 

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Once you have selected your lists, click the Display selection button to invoke the Venn diagram. 

 To save the current diagram displayed, click the Save image button. Images can be either in SVG or PNG format. You can also define the size and resolution you want the diagram to be. To maintain the fidelity of the colors in the Venn Diagram, we recommend downloading the image in PNG format. If a vector-based file format is required, you can also export the diagram in SVG format.

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