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  • Invoke the List Manager dialog by selecting Create Gene List in the Analysis section of the Gene Expression workflow
  • Ensure that the 1/ANOVA-3way (ANOVAResults) spreadsheet is selected as this is the spreadsheet we will be using to create our new gene list as shown (Figure 9)
  • Select the ANOVA Streamlined tab. In the  
  • Set Contrast: find genes that change between two categories panel, choose to Down Syndrome vs. Normal and select Have Any Change from the Setting dropdown  drop-down menu list. 

This will find genes with different expression levels in the different types of samples.

  • In the Configuration for “Down Syndrome vs Normal” panel, check that Include size of the change is selected and enter 1.3 into Fold change >  and -1.3 in OR Fold change <
  • Select Include significance of the change, choose unadjusted p-value from the dropdown menu, and < 0.001 for the cutoff. cutoff  

The number of genes that pass your cutoff criteria will be shown next to the # Pass field. In this example, 30 genes pass the criteria.

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  • Set Save the list as A, select Create, and then select 
  • elect Close to view the new gene list spreadsheet

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