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- Invoke the List Manager dialog by selecting Create Gene List in the Analysis section of the Gene Expression workflow
- Ensure that the 1/ANOVA-3way (ANOVAResults) spreadsheet is selected as this is the spreadsheet we will be using to create our new gene list as shown (Figure 9)
- Select the ANOVA Streamlined tab. In the
- Set Contrast: find genes that change between two categories panel, choose to Down Syndrome vs. Normal and select Have Any Change from the Setting dropdown drop-down menu list.
This will find genes with different expression levels in the different types of samples.
- In the Configuration for “Down Syndrome vs Normal” panel, check that Include size of the change is selected and enter 1.3 into Fold change > and -1.3 in OR Fold change <
- Select Include significance of the change, choose unadjusted p-value from the dropdown menu, and < 0.001 for the cutoff. cutoff
The number of genes that pass your cutoff criteria will be shown next to the # Pass field. In this example, 30 genes pass the criteria.
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- Set Save the list as A, select Create, and then select
- elect Close to view the new gene list spreadsheet
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