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  • Select Create Marker List from the Analysis section of the Illumina BeadArray Methylation workflow
  • Select anova-1way_autosomal (autosomal) as the source spreadsheet from the left-hand panel
  • Select LCLs vs. B cells from the Contrast: find genes that change between two categories section of the ANOVA Streamlined tab of the List Manager dialog
  • Set Fold change > to 20
  • Set OR Fold change < to -20
  • Set p-value with FDR to 0.005
  • Set file name as LCLs vs. B cells
  • Leave the rest of the option set to defaults (Figure 1)

 

Comment

May I suggest filtering by Estimate, instead? Fold-change is not typical for methylation studies.



Numbered figure captions
SubtitleTextCreating a marker list of differentially methylated genes between Naive shPOU5F1 and Naive shNANOG vs. Naive shCTRL
AnchorNameCreating a Marker List

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