PGS Documentation

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  • Select Tools from the main toolbar
  • Select Annotation Manager... (Figure 1)

 


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SubtitleTextSelecting Annotation Manager
AnchorNameSelecting Annotation Manager

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  • Select Browse... under Source to specify the BED file; a default new file name and destination will populate Result, but this can be changed 
  • You can specify the name and save location of the new annotation file under Result; we typically choose the Microarray Libraries folder  
  • Specify the Name of the annotation database file
  • Select the correct Species and Genome Build for the annotation file from the drop-down menus (Figure 3) 

 


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SubtitleTextConfiguring annotation file creation
AnchorNameConfiguring Annotation File Creation

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The Annotation Manager will display the new annotation in the My Annotations tab (Figure 4)

 

 

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SubtitleTextViewing created annotation in My Annotations
AnchorNameAdded Annotation

 

 

Visualizing a BED file as an annotation track in the genome browser

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  • Select Next >
  • Choose the annotation file you created; here we have selected UCSC CpG Islands (Figure 7)
  • If your annotation file does not contain strand information for each region, deselect Separate Strands; here we have deselected it

 

 

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SubtitleTextChoosing the annotation file
AnchorNameChoosing the annotation file

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SubtitleTextViewing the added annotation file as a track in the genome viewer
AnchorNameViewing the Annotation File in Genome Viewer

 

 

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