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- Select Tools from the main toolbar
- Select Annotation Manager... (Figure 1)
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- Select Browse... under Source to specify the BED file; a default new file name and destination will populate Result, but this can be changed
- You can specify the name and save location of the new annotation file under Result; we typically choose the Microarray Libraries folder
- Specify the Name of the annotation database file
- Select the correct Species and Genome Build for the annotation file from the drop-down menus (Figure 3)
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The Annotation Manager will display the new annotation in the My Annotations tab (Figure 4)
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Visualizing a BED file as an annotation track in the genome browser
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- Select Next >
- Choose the annotation file you created; here we have selected UCSC CpG Islands (Figure 7)
- If your annotation file does not contain strand information for each region, deselect Separate Strands; here we have deselected it
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