Alternative splicing results in a single gene coding for multiple protein isoforms, so this task can only be invoked from transcript level data. The algorithm is is based on ANOVA to detect genes with multiple transcripts showing expression changes differently in different biology groups, e.g. a gene has two transcripts: A and B,  transcript A is showing upreguation in treated group comparing to control group, and B is showing down regulation in treated group.

Alt-splicing ANOVA dialog

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