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By default, Trim bases removes bases starting at the 3' end and continuing until it finds a base pair call with a Phred score of equal to or greater than 20. Hover over the for additional information on any task option (Figure 2).
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The Trim bases task will generate a new data node, Trimmed reads (Figure 3). We can view the task report for Trim bases by double-clicking either the Trim bases task node or the Trimmed reads data node or choosing Task report from the task menu.
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- Double-click the Trimmed reads data node to open the task report
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The results are fairly consistent across samples with ~50% of reads untrimmed, ~45% trimmed, and ~5% removed for each. The average quality score for each sample is increased with higher average quality scores at the 3' ends.
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