Partek Flow Documentation

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After performing exploratory analyses such as PCA, UMAP and t-SNE is is helpful to visualize the results on a scatterplot. This can help visually assess the source of variation affecting the results of an experiment, classify cells and select samples for downstream analysis. Here we have a PCA scatterplot generated from the analysis of 12 samples from a scRNA sequencing study.


Numbered figure captions
SubtitleTextExample of a 3D PCA scatterplot .
AnchorNameExample of a 3D PCA scatterplot


The Configure > Style menu on the left can then be used to color the features in the scatterplot based on an attribute (Figure 2). In this case, Figure 3 shows the cells being colored based on their cell-type.


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SubtitleTextCustomization menu.
AnchorNameCustomization menu


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SubtitleTextPCA scatterplot colored by cell-type.
AnchorNamePCA scatterplot colored by cell-type.


Additionally, you can adjust the opacity of the points to better assess the density across the groups (Figure 4). It is also possible to split the plot based on the same attribute in the Configure > Grouping menu (Figure 5)


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SubtitleTextAdjusted opacity shows point density more accurately.
AnchorNameAdjusted opacity shows point density more accurately.


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SubtitleTextSplitting by an attribute can help better visualize their effect on the data.
AnchorNameSplitting by an attribute can help better visualize their effect on the data.



Click the Save image button  to save a PNG or SVG image to your computer. 

Click the Send to notebook button  to send the image to a page in the Notebook. 


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