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• Click the Normalized counts node
• Click Differential analysis Statistics in the task menu
• Click GSA Differential analysis in the task menu (Figure 1)
  
  

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Select the appropriate differential analysis method (Figure 2). In this tutorial we are going to use GSA, but Partek Flow offers a number of alternatives. Hover the mouse over the [...] symbol for more informations.

The Included attributes page shows all available attributes for analysis (Figure 23). Here, we only have one attribute, 5-AZA Dose.

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The Comparisons page will open (Figure 34). 

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• Select 5μM in the upper box
• Select 0μM in the lower box 
• Click Add comparison to add 5μM vs. 0μM to the comparison table (Figure 45)
  
  

• Repeat to create comparisons for 10μM vs. 0μM and 5μM:10μM vs. 0μM (Figure 56)
  
  

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A GSA task node and a GSA data node will be added to the pipeline (Figure 67). 

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