Partek Flow Documentation

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Terminology 

Data Viewer - a collection of sheets

Sheet - view one or more linked plots and controls

Plot - a visualization

Icon - invokes modification controls 

Menu - icons located on the left side of the data viewer

Droppable - data that can be dragged and dropped 

Target - location to drop a droppable 

Node - Image Modifieddata results from the analysis pipeline

FAQs

Q- What is the Data viewer? 
A - The Data viewer is a general-purpose data visualization tool that includes 2D and 3D scatter plots, histograms, heatmaps, violin plots, and more. 

Q- How is it invoked? 
A- Select a data node and double-click to view the task results in the Data viewer. You can also select the Data viewer tab in a project to start a new session or revisit a saved session. 

Q- Can I view data from multiple data nodes? 
A- You can view data from different nodes in one sheet, but not in one plot. 

Q- Can I open more than one icon at a time and move it around?
A- Yes, open as many icons as you need at a time and move them around the screen. Click Image Modified in the right corner to close the icon. 

Q- Is there a recently used function?
A- Yes, there is a recently used function within the Get data icon. This lets you drag recent nodes or the green droppables to blue targets. 


How do I get started and navigate the data viewer?

To get started in a new data viewer session click New session Image Modifiedor open a previously saved session. Clicking New session will prompt you to drag and drop an available plot or data node to visualize your data onto the sheet. To do this, use New plot or Get data under Setup in the left menu. The menu descriptions can be shown Image Modifiedor hidden Image Modified as desired. The menu is organized into single-click common controls, Setup, Tools, and Configure icons. Common controls and Setup icons do not change but Tools and Configure icons are context-sensitive and will change according to the selected plot type. Multiple icons can be open at once and their dialogs dragged around the screen as desired. Close the dialog by clicking the Image Modified in the right corner. 

  • Common controls:
    Image ModifiedSave - overwrite a saved session
    Image Modified Save as - save a new session using a new name
    Image Modified Undo - recover backward actions
    Image Modified Redo - recover forward actions
    Image Modified Export image - download one plot of all plots in a view to your computer
    Image Modified Send to notebook - save an image of the entire sheet (all of the plots in view) to the notebook

  • Setup:
    Image Modified New plot - icons used to make a new plot
    Image Modified Get data - select data from the analysis pipeline
  • Tools:
    Image Modified Select & filter - make selections and filter 
    Image ModifiedClassify - modify and apply selections as classifications 
    Image Modified Additional actions - create a feature list 

  • Configure icons are context-sensitive and available options will change according to the plot type selected. Please navigate to the Configure help section below for more details. 


How do I make a plot?

To make a new plot in the Data viewer, use the Setup icons.

Image ModifiedNew plot - Clicking a plot type will suggest appropriate data options from results available in the analysis pipeline, then click the node of interest to add it to the sheet. Alternatively, drag and drop the plot to the sheet and continue with any additional selections. 

                  Image Modified


Image ModifiedGet data - Choosing data will suggest appropriate plot types once the data is dragged onto the sheet. Note that All data nodes (left) follow the hierarchy used in the analysis pipeline while Recently used (right) nodes are updated according to recent use and can similarly be used to plot data. 

                Image Modified


Note the target locations presented in blue (top, bottom, left, right, & replace) when dragging data. In this example, a data node chosen from Get data, is being dragged onto the sheet and it can be placed on the Top, Right, Bottom, Left, or Replace the existing heatmap. Once dropped, a plot type must be chosen. 

                Image Modified


Can you have multiple plots on the sheet?

Yes, build them together. 

  • All plots are interactive. 
  • All plots are from the same data project. 
  • Selection (on the right side of individual plots Image ModifiedImage ModifiedImage ModifiedImage Modifiedand in the menu Image Modified) and filtering options are linked for the plots on the sheet. Clear filters Image Modifiedin the Select & Filter Image Modified mode to remove selections. 
  • Save plots together from the menu Image ModifiedImage Modified or independently within each plot Image ModifiedImage Modified

When there are multiple plots, how are they controlled and configured?

  • Each plot has its own window which can be resized by dragging the edge of the plot. 
  • Click a plot window to make it active (a dark gray border will appear for the window). The Configure icons will show settings for the active plot. 
  • When more than one plot is open on a sheet, plots can be rearranged by dragging from one position to another Image Modified, duplicated Image Modified, enter full screenImage Modified, and exit full screenImage Modified
  • If multiple plot types are selected together with ctrl-click or shift-click, only the plot configuration controls that are shared will be available for use. 
  • Image Modified Select & Filter - applies to all of the plots on the sheet. 
  • Controls are located in the same place for each plot, either in the menu on the left, the top of the plot, or the right side of the plot. Even though each plot type has its own available controls that will vary, common controls such as those available above Setup in the menu: 

...

At the left of the plot:
Image Added Save

...


Image Added Save a
Image Added Undo
Image Added Redo
Image Added Export image
Image AddedTo notebook

  • At the top of the plot:
    Image Added Hide controls

...


  • Image AddedShow controls
    Image Added Click and drag to move the plot

...


  • Image Added Duplicate

...


  • Image Added Export image

...



Image Added Send to notebook

...


Image AddedEnter Full screen,
Image Added Exit Full screen
Image Added Remove plot 

My screen is full of plots. How do I add more plots without starting a new data viewer session? 

Multiple sheets (where plots are added) are allowed in the data viewer. This is helpful when you cannot fit more plots on the sheet (screen) but you still want to use all of the settings that you are working with (e.g. recently used).

  •  Sheet navigation uses a panel at the bottom of the screen Image Modified. Use Image Modified to Add a sheet or the Image Modified to Duplicate, Rename, or Delete a sheet.  

Why does some data light up green when I hover over it? 

Any data highlighted in a green background Image Modified when hovered over can be dragged and dropped onto a blue target Image Modified.

  • This includes legend, axis titles, criteria used in Select & Filter, and table features. Any data present in Plot data will be highlighted in green and can be dragged to make a plot.
  • Drag and drop manipulation is also possible for data nodes Image Modifiedpresent in the configure icons (e.g. Axes and Style).

How do I color my plot by a gene? 

There are multiple ways to color plots by a gene or protein (feature) of interest. You can also color by more than one feature on the same plot or even feature lists. You can also choose to represent a feature with styles other than color, like shape or size.  

  • Drag and drop the gene of interest from the appropriate node in Get data (e.g. normalized counts). The Recently used list can be helpful for this if you have already recently used the gene. 
  • Open the Style icon and change Color by. Make sure you are on the appropriate node Image Modified. Some plot types (e.g. tSNE) will invoke Style by clicking the legend. This is where you would color by a feature list that you have saved under list management. 
  • Drag and drop features from another plot on the sheet (e.g. an axis title from a violin plot) or drag and drop features from criteria added to the Select & Filter icon. 

Can I plot genes from tables without going back to the analysis pipeline? 

Yes, report results such as those generated during differential analysis can be accessed in the data viewer and specific genes can be plotted. 

  • The tables must be plotted on the sheet. One way to do this is to choose New plot Image Modified, select Table Image Modified (only data in table format are available), then the data to plot. Features (green with hover) are now available to drag and drop on the plot type of interest. 

How do I manipulate my plot without using Tools or Configure? 

  • Selections can be made directly on the plot  Image ModifiedImage ModifiedImage ModifiedImage Modified. This can include selecting populations of cells or selecting genes of interest (e.g. to classify clusters of cells). The menu must still be used to filter Image Modified, classify Image Modified, and create gene lists Image Modified after in-plot selection. 
  • Clicking on the Legend will invoke configure options. The configure options invoked will vary depending on the plot type. 
  • Clicking the axis titles will invoke the axes configuration. 

Setup 

Image Modified New plot

  • Click a plot typeChoosing a plot type will suggest appropriate data options from results available in the analysis pipeline. The search bar can be used to navigate to a specific node of interest (e.g. normalized). Select a data node to add it to the sheet. 
  • Alternatively, drag and drop the plot to the sheet (blue targets) and continue with any additional selections.  
  •          Image Modified

Image Modified Get Data

All data results from building the analysis pipeline are found hereThis icon is used to create plots, to plot attributes and features of interest (e.g. color a plot by a gene of interest from the normalized data node), and to plot Recently used data nodes and droppables. 

  • Find the data node by expanding the pipeline or use the Search bar to quickly locate results. 

  • To create a plot, drag the node to the sheet and continue with additional selections. 
  • Recently used (right side) data is organized according to the node from which it is derived and will update according to recent use. If the data viewer session is saved, recently used will be saved.
  • Click a node under All data to search data found within that node such as attributes, genes, or lists. Drag data (green droppable) to blue targets (e.g. search the normalized counts node for a gene then drag the gene to color a tSNE plot). Numeric attribute names (e.g # cells) are green while categorical attribute names (e.g. classifications) are blue. 

                 Image Modified


Tools

Image Modified Select & Filter

This icon is where selections are made (left side) and filtered (right side). Selecting and filtering will be applied to all plots in the sheet. Selections can be used to filter and/or classify Image Modified. Selections used to filter can be named under classify Image Modifiedand indicate the number of cells included. 

  • Deselected points (the points that are not selected) can either be Dim (colors are not as bright) or Gray (not colored but instead gray)
  • There are two selection modes: Manual and Criteria.
    • Manual
      • Manual governs in plot selections Image ModifiedImage ModifiedImage ModifiedImage Modified which can undergo filtering or classification. 
    • Criteria
      • Criteria allows additions according to the selected node Image Modified(this is criteria used to configure the plot like attributes or genes). 
      • Data can be dragged directly to Add criteria to generate selections or use the drop-down. 
      • When criteria are added, the icon will grow with the selections. Remove the selections by clicking Image Modified
      • Selections can be dragged to blue targets. 
      • Each criteria can be modified. Invert can be used to include or exclude selections. When modifying criteria by low or high values, pin histogram lets you see multiple histograms at a time. Histograms are colored according to selections: the dark blue is what’s being selected for, the gray is the data outside the current selection (i.e. the positions of the slider handles), and the light blue is data that is within the slider’s selected region but is currently deselected by other selection criteria. 

                                               Image Modified

  • Filter 
    • Click Image Modifiedto include the selected points, click Image Modifiedto exclude the selected points, and click Image Modifiedto remove filters. 
    • Apply observation filter and Apply feature filter are used to apply filters to data nodes in the analysis pipeline. 

                                            Image Modified


 Image Modified Classify

The Classify icon holds draft classifications to be applied or saved classifications that can be opened, applied, and refined as necessary then saved again as a new classification. Classification names can be edited and the Classifications can be deleted. The number of points in the classification are listed and will be updated accordingly with any changes. Classify selection requires a label (name) and will overwrite the previous applied classification if the points have already been classified. Apply classifications can be used to apply the classification to a data node. 

               Image ModifiedImage Modified


Image Modified Additional actions

The Additional actions icon holds other available plot actions. This icon will become available for applicable plot types (e.g. heatmap). Currently, it is used to Create a feature list.  

Configure 

These are the configuration options for plots. Open as many icons as you need and move them around the screen then click Image Modified in the right corner to close the icon when finished. Changes will automatically be saved before closing and will not affect other plots unless both windows are selected and modified together. If an icon is opened from a plot and kept on the screen but not available for use in a different type of plot, you will be notified that the configuration is not available for the selected plot. 

Image Modified Description

  • Click the Title on a plot or choose the Description icon from the menu. 
  • This contains Title and Legend settings. 

Image Modified Axes

  • Click the Axes icon from the menu. The axes dialogue can also be opened by clicking the X, Y, or Z axis titles. 
  • The available options change depending on the plot type. 
  • Features and attributes can be dragged to the Axes card Image Modified. Alternatively, drag feature and attributes from the axis to plots using the data node Image Modified

Image Modified Data

  • Data configuration options frequently change for different plot types. See the examples below. 

                Image ModifiedImage ModifiedImage ModifiedImage Modified

Image Modified Style

  • Style can be invoked from the menu or by clicking the Legend of most plot types. 
  • This is where Color, Size, Shape, Labeling, and Style (bar or line) settings are found. 

Image Modified Grouping

  • Grouping is only invoked from the menu. 
  • This is where you split, connect, and highlight by data. 

Image Modified Statistics

  • Statistics is invoked in the menu. 
  • It is used for Analytics (regression line) and to modify plot Significance

Image Modified Background

  • The Background can be modified for some ploy types; this setting is found in the menu. 
  • PNG, JPG, and BMP files are supported by all major web browsers. Your browser may support additional formats. 

Image Modified Control 

  • Control is used for 3D scatter plots and found in the menu. 
  • This is where settings for moving the 3D plot are found. 

Heatmap & Bubble map 

Please see Hierarchical Clustering Configuration Settings for details related to configuring a heatmap or bubble map in the data viewer.





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