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There will be no inputs needed if the FASTQ is converted to counts matrix within Flow. However, if users processed the FASTQ files outside of Partek, and imported the counts matrix into Flow later. The feature_linkage.bedpe file in outs/analysis/feature_linkage from Cell Ranger output will be needed for each sample (Figure 2) to complete the analysis.

For data where no sample attributes are specified, the peak detection pairs need to be manually defined. In the example in Figure 1, there only two samples. Under the Define pairs section, the left panel lists all the sample names uploaded to the project (H3K27 and Mock).  Add one pair at a time by dragging the corresponding samples to either the IP panel on the top-right or the Control panel on the bottom-right. If no control samples are present in the experiment, leave the Control panel blank. If more than one ChIP or Control samples are added, the samples will be combined (or pooled) during the analysis. After defining a pair, click the Add pair button.

For data where the sample attributes are defined (Figure 3), you will have an additional option to add pairing or grouping based on sample attributes. Figure 3 shows an example dataset with 4 samples, 2 time points, and there is one IP sample and one Input sample in each time point.

When running the MACS2 task, sample attributes will be used to define the multiple pairs (Figure 4). There is an IP-Input pair for each time point, so the Pair attribute is the Time attribute. The Control attribute is the attribute that differentiates between the Input and IP groups, and in this example, it is the ChIP attribute.  Finally, the Control term is labeled as Input in the example. 

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If multiple pairs are added in the Pairs table, the peak detection is performed on each pair independently.

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Task report

In the task report, each pair will generate a list of peaks displayed in a table (Figure 6). Use the drop down menu next to Peaks detected for... to select the pair.

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