Partek Flow Documentation

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SubtitleTextRNA-Seq workflow with principal components analysis (PCA) task invoked on the Gene counts data node
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On the other hand, the Cufflinks quantification node is not associated with a detailed report like the Partek E/M nodes, so opening the node by double clicking (or going via the toolbox) brings up the PCA plot directly.

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SubtitleTextPrincipal components analysis plot in 3D. Each dot is a sample and samples originating from the same biological source (dependent study design) are connected by lines. The axes show the first three principal components, with the fraction of explained variance in the parenthesis
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If you want to change a color, select the Customize colors hyperlink. The resulting dialog (Figure 5) will enable you to replace an existing color by a color of your choice (click on the arrow head to invoke the color mixer) or add more colors (Add color).

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SubtitleTextPrincipal components analysis plot in 2D. Each dot is a sample. The axes show the first two principal components, with the fraction of explained variance in the parenthesis (an example)
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Although first three PCs are shown by default, you can plot any of the first nine PCs, by using the X, Y, and Z drop-down lists.

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