Color by: the options control colouring of Alignments and Isoform proportion tracks. Sample, Base, and Match options are present by default. If attributes have been assigned to samples, they will appear in the drop-down list. In this example, that is the "Tissue" attribute
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Color options
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Difference between Color by Sample and Color by <Attribute>. Color by Sample uses different colours to depict individual samples; Color by <Attribute> uses different colours to depict levels of the selected sample attribute (as present in the Data tab). Alignments and Isoform proportion tracks are shown (an example)