Flow Documentation

Partek Flow Documentation

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As a prerequisite for invoking the volcano plot, you must run a Differential gene expression analysis. When setting-up the analysis, include all relevant contrasts you would like to view the volcano plots for.

Once the analysis is complete, open the resulting Feature list data node. Navigate to the Gene list section of the page and click on the volcano plot icon, located above the header of a Fold change column (Figure 1).  

 

Numbered figure captions
SubtitleText Invoking a volcano plot from a Feature list data node
AnchorNameinvoke-volcano-plot

Each point on the plot represents the statistical result for a single gene, transcript or feature. The color of the point reflects fold change: genes with a fold change above 2 (up-regulated) are in green, genes with a fold change below -2 (down-regulated) are in red. The remaining genes are in grey (Figure 2). The plot header is derived from the name of the contrast.

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