Partek Flow Documentation

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SubtitleTextUse the Configuration cards on the left customize the appearance of plots
AnchorNameConfigure single cell QA/QC plot

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High-quality cells can be selected using the Selection card on the right, which is pre-loaded with three selection rules, one for each quality metric (Figure 5).

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SubtitleTextThe Selection card with three selection rules
AnchorNameSelection card Single cell QAQC

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Hovering the mouse over one of the selection rules reveals a histogram showing you the frequency distribution of the respective quality metric. The minimum and maximum thresholds can be adjusted by clicking and dragging the sliders (Figure 6).

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SubtitleTextUse the sliders and histograms to adjust the selection criteria
AnchorNameSelecting cells using sliders

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Alternatively, the thresholds can be set by typing directly into the text boxes in each selection rule (Figure 7).

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SubtitleTextMinimum and maximum thresholds can be set by manually typing into the text boxes
AnchorNameSelecting cells using text boxes

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Adjusting the selection criteria will select and deselect cells in all three plots simultaneously. Depending on your settings, the deselected points will either be dimmed or gray. The filters are additive. Combining multiple filters will include the intersection of the three filters. The number of cells selected is shown in the figure legend of each plot (Figure 8).

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SubtitleTextThe figure legends show 3696/4352 cells with total count <12,000, number of detected features 600 - 2500, and % mitochondrial count <5% are selected in all three plots. Selected cells are blue, deselected cells are dimmed.
AnchorNameSingle cell QAQC with selection criteria applied

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To filter the high-quality cells, click the  icon in the Filtering card in the top right, and click Apply observation filter... (Figure 9).

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SubtitleTextInclude the selected points and apply the filter
AnchorNameApply filter

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Select the input data node for the filtering task and click Select (Figure 10).

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