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Seurat3 integration in Flow can be invoked in Batch removal section if a Normalized counts data node is selected (Figure 1).
Figure 1. Seurat3 integration task in Batch removal section in Flow.
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- Select Tech from the dropdown list
- Click Finish
Figure 2. Interface of Seurat3 integration in Flow. Example attributes are indicated in the drop-down list.
The output of Seurat3 integration is a new data node - Integrated counts (Figure 1). We can then use this new integrated matrix for downstream analysis and visualization (Figure 3).
Figure 3. UMAP displays the cells colored by Tech attribute before(left) and after(right) Seurat3 integration. Both images in the figure share the same legend.
Users can click Configure to change the default settings In Advanced options (Figure 4).
Figure 4. Advanced configure options for Seurat3 integration in Flow.
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