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If you want to specify sample IDs instead of using hashtag feature IDs as the sample IDs, you will need to prepare a Sample ID tab-delimited .csv txt file with hashtag feature IDs in the first column (Hashtag) and the corresponding sample IDs in the second column (Sample) (Figure 1). 


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SubtitleTextSample ID .csv file example. The first column is the hashtag feature ID and the second column is the corresponding sample ID
AnchorNameSample ID file

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Running Hashtag demultiplexing

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The output is a Dumultiplexed counts data node (Figure 2).

 


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SubtitleTextHashtag demultiplexing can be run on a Normalized counts data node and outputs a Demultiplexed counts data node
AnchorNameHashtag demultiplexing output

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  1. Stoeckius, M., Zheng, S., Houck-Loomis, B., Hao, S., Yeung, B.Z., Mauck, W.M., Smibert, P. and Satija, R., 2018. Cell Hashing with barcoded antibodies enables multiplexing and doublet detection for single cell genomics. Genome biology, 19(1), p.224.

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