Partek Flow Documentation

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This produces a Filtered groups data node (Figure ).

 

 

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SubtitleTextFilter groups output
AnchorNameFIlter groups output

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This will produce two data nodes, one for each data type (Figure ).

 

 

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SubtitleTextSplit matrix can also re-split the data
AnchorNameSplit matrix

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  • Click Finish to run the statistical test (Figure )

 


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SubtitleTextSetting up a comparison in the GSA task
AnchorNameAdding comparisons

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The report lists each feature tested, giving p-value, false discovery rate adjusted p-value (FDR step up), and fold change values for each comparison (Figure ).

 

 

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SubtitleTextGSA report for the protein expression data
AnchorNameGSA report

In addition to the listed information, we can access dot and violin plots for each gene or protein from this table.

 

  • Click 
  • Image Modified

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  •  in the 
  • CD25_TotalSeqB 

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  • row

This opens a violin plot showing CD25 expression for cells in each of the classifications (Figure ).

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The number at the top of the filter will update to show the number of included genes (Figure ).

 

 

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SubtitleTextFiltering GSA results to significant genes
AnchorNameFiltered GSA results

 

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