Partek Flow Documentation

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SubtitleTextCell barcode QA/QC task report is used to filter barcodes
AnchorNameTask report for Cell Barcode QA/QC

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The knee plot is used to choose a cutoff point between barcodes that correspond to cells and barcodes that do not. Partek Flow automatically calculates a cutoff point, shown by the vertical line on the graph. Barcodes designated as cells are shown in blue while barcodes designated as without cells (background) are shown in grey.

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To view a summary of the currently selected filter settings for all samples, click Summary table. This opens a table showing key metrics for each sample in the project (Figure 2).

 

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SubtitleTextBarcode QA/QC summary table lists filtering information for each sample
AnchorNameSummary table for Barcode QA/QC

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To return to the knee plot view, click Back to filter. To apply the filter and run the filter barcodes task, click Apply filter

 

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