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Normalization can be performed by sample or by feature. By sample is selected by default; this is appropriate for the tutorial data set.
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For this tutorial, we will use the recommended default normalization settings.
- Select
This adds Total count CPM (counts per million) and Add 0.0001 to the Normalization order panel (Figure 3). Normalization steps are performed in descending order
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Total Count CPM (counts per million) normalizes read counts for each gene by the total count of the sample. This accounts for differences in total read counts between samples.
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Overview
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