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• Linux: Ubuntu^® 12.04, Redhat^® 6, CentOS^® 6, or newer
• Mac: OS X Yosemite 10.10 or newer
• 64-bit 2GHz quad-core processor¹
• 48GB of onboard memoryRAM²
• 1TB of storage available for data
• /opt – 100GB of storage available for installation

• A broadband internet connection

¹Note that some analyses have higher requirements, e.g. system requirements for example to run the STAR aligner on a reference genome of size ~3 GB (such as human, mouse or rat), 16 cores are required.

²Input sample file size can also impact memory usage, which is particularly the case for TopHat alignments. For using our Single Cell Toolkit, analysis of multiple samples with over 5000 cells per sample will require at least 64 GB of memory.

Increasing hardware resources (cores, RAM, disk space, and speed) will allow for faster processing of more samples.

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