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- Click the Single cell data node produced by the Filter cells task
- Click Filtering in the task menu
- Click Filter features (Figure 20)
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There are three categories of filter available - Noise reduction filters, Statitics bsaed filters, and Feature list filters (Figure 21).
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We will use a Noise reduction filter to exclude genes that are not expresed by any cell in the data set, but were included in the matrix file.
- Click the Noise reduction filter check box
- Set the Noise reduction filter to Exclude features where expression value == 0 in 100% of cells using the drop-down menus and text boxes (Figure 22)
- Click Finish to apply the filter
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This produces a Filtered counts data node. This will be the starting point for the next stage of analysis - identifying cell types in the data using the interactive t-SNE plot.
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