...

• Activate the 3D lasso tool by selecting 
• Draw the lasso around the cluster of red cells and click the circle to close the lasso (Figure 27)
  
  

• Select Save classifications

These red cells are MOBP positive, indicating that they are the oligodendrocytes from every sample. 

...

• Switch modes by selecting 
• Deselect by clicking on any black space on the plot

Red Green CD14 positive cells are the microglia/macrophages from every sample. 

• Activate the 3D lasso tool by selecting 
• Draw the lasso around the cluster of green cells and click the circle to close the lasso (Figure 28)
  
  

• Select Save classifications
• Name the classification Microglia
• Select Save
• Switch modes by selecting 
• Deselect by clicking on any black space on the plot

...

• Activate the 3D lasso tool by selecting 
• Draw the lasso around the grey cells and click the circle to close the lasso (Figure 29)
  
  

• Select Save classifications 
• Name the classification Malignant
• Select Save

...

The pipeline view will open and the Classify cells tasks will run, generating a new green-layer Classified groups data node (Figure 2730).