The significant CpG loci detected in the previous step actually form a methylation signature that differentiates between LCLs and B cells. We can build and visualize this methylation signature using clustering and a heat map.

• Select the LCLs vs. B cells _vs_Bcells_CpG_Islands spreadsheet in the spreadsheet pane on the left
• Select Cluster Based on Significant Genes from the Visualization panel of the Illumina BeadArray Methylation workflow
• Select Hierarchical Clustering for Specify Method (Figure 1)
  
  

• Select OK
• Verify that LCLs vs B cells is _vs_Bcells_CpG_Islands is selected in the drop-down menu
• Select Verify that Standardize is selected for Expression normalization (Figure 2)
  
  

• Select OK 

The heat map will be displayed on the Hierarchical Clustering tab (Figure 3).

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