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• Select Make a pipeline near the bottom left-hand side of the browser window
• Select Tim Select the Tim bases, Pre-alignment QA/QC, Align  Align reads, Quantify to annotation model, Post-alignment QA/QC, Normalize counts, PCA, and Gene analysis task nodes to include them in the pipeline
• Name the pipeline; we have chosen RNA-Seq basic analysis 
• Give a description for the pipeline; we have noted trim <20, STAR, normalize with total count and add 0.001, GSA 
• Select Make pipeline (Figure 1)
  
  

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