By following the steps in this tutorial, you have built a pipeline. You can save this pipeline for future use.
- Select Create new pipeline near the bottom left-hand side of the browser window
- Select the Pre-alignment QA/QC, Trim bases, Align reads, Post-alignment QA/QC, Quantify to annotation model, Filter features, Normalize counts, PCA, and GSA task nodes to include them in the pipeline
- Name the pipeline; we have chosen RNA-Seq basic analysis
- Give a description for the pipeline; we have noted trim <20, STAR, normalize with total count and add 0.001, GSA
- Select Create pipeline (Figure 1)
Figure 1. Select tasks to include then provide a name and description for the new pipeline
To access this pipeline in the future, select an unaligned reads data node and choose Pipelines from the task menu. Available saved pipelines will be available to choose from the Pipelines section of the task menu (Figure 2).
Figure 2. Created pipelines will appear in the Pipelines section of the task menu
After selecting the pipeline, you will be prompted to choose the reference genome for alignment, the annotation for quantification, and the contrasts for GSA. After selecting these options, the pipeline will automatically run. See Pipelines for more information.
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