Partek Flow Documentation

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From the GSA task report, we can browse to any gene in the Chromosome Viewview.

  • Select  in the SELM row to open Chromosome View view (Figure 1)

Numbered figure captions
SubtitleTextBrowsing to a location in Chromosome View
AnchorNameBrowsing to location

 Image Added

A new tab will open showing SELM in the Chromosome View view (Figure 2).

 

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SubtitleTextViewing SELM in Chromosome view
AnchorNameSELM in Chromosome view

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Chromosome View shows reference genome, annotation, and data set information together aligned at genomic coordinates. 

Each track has Configure track  and Move track  buttons that can be used to modify each track. 

The top track (Figure prev.) shows average number of total count normalized reads for each of the three treatment groups in a stacked histogram. The second track shows the RefSeq annotation.

We can add tracks from any data node using Select Tracks.

 

  • Select Select tracks

A pop-up dialog showing the pipeline allows us to choose which data to display as tracks in Chromosome Viewview (Figure 3).

 

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SubtitleTextChoosing tracks to display in Chromosome view
AnchorNameChoosing tracks to display

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  • Select Reads pileup under Align reads on the left-hand side of the dialog 
  • Select Display tracks to make the change

The reads pileup track is now included (Figure 4).

 

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SubtitleTextThe reads pileup track shows every read in its aligned position on the reference genome
AnchorNameReads pileup track

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With multiple tracks, it may be useful to pin a track to the top so we can scroll down the reads pileup track without losing sight of the Alignments or RefSeq tracks. 

  • Select Image Added next to the Alignments track
  • Select Image Added to pin the track to the top 
  • Repeat for RefSeq
  • Scroll down to view individual reads 

Selecting a read brings up detailed information about the read in the selection details panel (Figure 5).

 

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SubtitleTextViewing read information in the Reads pileup track
AnchorNameViewing read information

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To learn more about Chromosome Viewviewplease consult the Chromosome View user guide.

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