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• In the Configure Criteria dialog box (Figure 2), provide a name for the list (Diff Exp)
• Select 1/transcripts (RNA-Seq_results.transcripts) from theSpreadsheet drop-down menu 
• Select 8. p-value(DiffExp) from the Column drop-down menu
• Set Include p-values to significant with FDR with a value of 0.05

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• Select OK to generate Alt Splice
• Select both lists in the right-hand panel under the Criteria panel while holding the Ctrl key on your keyboard
• Select Intersection from the left-hand panel of the List Manager dialog (Figure 4)
  
  

• Enter a name for the criteria (Diff Exp and Alt Splice) 
• Select OK to close the naming dialog and OK again to close the list creation hint dialog
• Select Save List from the Manage criteria section of the List Manager dialog (Figure 5)
  
  

• Select Diff Exp and Alt Splice in the List Creator dialog (Figure 6)
  
  

• Select OK to save the list
• Select Close to exit the List Manager dialog and view the Diff_Exp_and_Alt_Splice spreadsheet (Figure 7)
  
  

This list of differentially expressed and alternatively spliced transcripts will be used later in the next section of the tutorial. 

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