Flow Documentation

Partek Flow Documentation

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Numbered figure captions
SubtitleTextERCC spike-ins plot. Lines (one per sample) correspond to regression lines between actual spike-in concentrations and observed number of alignments. Dots represent present ERCC sequences with the lowest and the highest concentration
AnchorNamespike-ins

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Optionally, you can invoke a principal components analysis plot (View PCA), which is based on RPKM-normalised counts, using the ERCC sequences as the annotation file (not shown).

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SubtitleTextScatter plot of actual observed alignment counts vs. probe concentration for each ERCC control within a sample. Each dot is an ERCC control, coloured by GC content and sized by concentration
AnchorNamescatter-plot

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The table (Figure 7) lists individual controls, with their actual concentration, alignment counts, sequence length, and % GC content. The table can be downloaded to the local computer by selecting the Download link.

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