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The Create categorical attribute dialog (Figure 3) allows us to create groups for a categorical attribute. By default, two groups are created, but additional groups can be added.
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- Set Attribute name: to State
- Rename the groups Primed and Naive
- Drag and drop the samples from the Unassigned list to their groups as listed in the table below
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There should now be two groups with samples in each group (Figure 4).
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- Select OK
- Select Yes from the Add another categorical attribute dialog
- Set Attribute name: to shRNA treatment
- Select New Group to add an additional group
- Rename the three groups Control, shPOU5F1, and shNANOG
- Drag and drop the samples from the Unassigned list to their groups as listed in the table below
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