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During a previous section of this tutorial, a spreadsheet named unexplained_regions was generated. This spreadsheet contains locations where reads map to the genome but are not annotated by the transcript database, in this case, RefSeqGene. TheunexplainedThe unexplained_regions spreadsheet regions spreadsheet is potentially very interesting as it may contain novel findings.

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Please note that it is recommended that you annotate with the same database as used when you performed mRNA quantification.

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