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To identify transcripts that are both significantly differentially expressed AND alternatively-spliced among the four tissue samples, we can use the Create Gene List function.

• Select Create Gene List from the Analyze Known Genes panel of the RNA-seqSeq workflow to invoke the List Manager dialog 
• Select the Advancedtab (Figure 1)
  
  

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• Enter a name for the criteria (Diff Exp and Alt Splice) 
• Select OK to close the naming dialog and OK again to close the list creation hint dialog
• Select Save List from the Manage criteria section of the List Manager dialog (Figure 5)
  
  

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• Select OK to save the list
• Select Close to exit the List Manager dialog and view the Diff_Exp_and_Alt_Splice spreadsheet (Figure 7)
  
  

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