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 In this tutorial, the file name (e.g., Down Syndrome-Astrocyte-748-Male-1-U133A.CEL) contains the information about a sample and is separated by hyphens (-). Choosing to split the file name by delimiters will separate the categories into different columns as shown (Figure 8).

  • In the Sample Information panel, specify the column labels (Labels 1-4) as TypeTissue, Subject, and Gender, set each as categorical, and set the other columns as skip (Figure 8). Select OK

Numbered figure captions
SubtitleTextConfiguring the Sample Information Creation dialog
AnchorNameSample Information Creation

  • A dialog window asking if you would like to save the spreadsheet with the new sample attribute will appear. Select Yes
  • Make column 5. (Subject) random by right-clicking on the column header and selecting Properties from the pop-up menu (Figure 9). 

Numbered figure captions
SubtitleTextChanging column properties
AnchorNameChanging Column Properties

  • Select the Random Effect check box from the Properties dialog (Figure 10) then select OK. 

Numbered figure captions
SubtitleTextSetting column to Random Effect
AnchorNameProperties of Column

The column 5. (Subject) will now be colored red, indicating that it is a random effect. 

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