Partek Genomics Suite enables you to visualize each probe and compare the methylation between the groups at a single CpG site level.
- Right click row 19 Cdk14 in the LCLs vs. B cells spreadsheet
- Select Browse to Location from the pop-up menu
- RefSeq Transcripts 2014-01-03 (hg19) (+): transcripts coded by the positive strand
- RefSeq Transcripts 2014-01-03 (hg19) (-): transcripts coded by the negative strand
- Regions: by default, fold-change difference in methylation between the groups
- Heatmap: M values for all the samples
- Barchart: methylation levels of the selected sample (to select a sample, click on a heat map)
- Cytoband: cytobands of the current chromosome
- Genomic Label: coordinates on the current chromosome
To modify a track, select it in the Tracks panel to bring up its configuration options panel below the Tracks panel. Let's modify a few tracks to improve our visualization of the data.
- Select the Regions track, opens to Profile tab
- Set Bar height by to Estimate
- Set Min and Max to -10 and 10
- Select Apply to change
- Select Color tab
- Set Color bars by to Fold Change (LCLs vs. B cells) (Description)
- Select Apply to change
This will allow us to visualize the differences in methylation between the two groups.
- Select the Bar Chart track, opens to Y-axis tab
- Set Min and Max to -5 and 5
- Select Apply to change
- Select a sample to display in the Bar Charttrack by left-clicking on a row in the heat map
A bar indicating the M value at this probe set will appear in the Bar Chart track (Figure 3)
The available tracks can be supplemented with a special annotation file that can be built using a UCSC annotation file as the basis. Building and viewing the UCSC annotation file is available as an optional section of the tutorial, Optional: Adding UCSC CpG Island Annotations.
Additional Assistance
If you need additional assistance, please visit our support page to submit a help ticket or find phone numbers for regional support.
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