In this tutorial, we demonstrate how to:
The tutorial is based on the work published by Venteicher and co-workers, on isocitrate dehydrogenase-mutant gliomas. Single cells from tumor biopsies were processed by flow cytometry and the libraries were prepared by Smart-seq2 protocol. The tutorial data set consists of eight expression matrix files, one per patient sample. The tumors were categorized as either astrocytoma or oligodendroglioma glioma subtype by histology. The matrix files contain gene expression values normalized by the following transformation log2[(TPM/10)+1].