Attributes are used to describe your samples. Examples of sample attributes include treatment group, age, sex, and time point. Attributes can be added manually or using a tab-delimited text file with samples on rows and attributes on columns where the first column contains the sample IDs. In this tutorial, we will add one attribute, Treatment, manually.

• Select the Data tab
• Select Manage attributes (Figure 1)
  
  

• Select Add new attribute (Figure 2)
  
  

• Set Treatment as Name
• Select Categorical as Attribute type 
• Select Project-specific for Visibility 
• Select Add (Figure 3)
  
  

• Name the first New category 0uM
• Select Add
• Name the second New category 5uM
• Select Add
• Name the third New category 10uM
• Select Add
  
• Select Close to finish creating the attribute (Figure 4)
  
  

• Select Back to sample management table 

The data table now includes an Attribute column for Treatment (Figure 5). Next, we need to assign samples attribute categories for Treatment.

• Select Edit attributes

The option to edit the Treatment field for each sample will appear as a drop-down menu (Figure 6).

• Select the Treatment text box for a sample to bring up a drop-down menu with the Treatment attribute categories (0uM, 5uM, 10uM)
• Use the drop-down menus to add a treatment group for each sample

The first three samples (SRR592573-5) should be 0uM, the next three samples (SRR592576-8) should be 5uM, and the final three samples (SRR592579-81) should be 10uM (Figure 7).

The data table will now show each a Treatment attribute for each sample.