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To learn more about the biology underlying gene expression changes, we can use gene ontology (GO) or pathway enrichment analysis. Enrichment analysis identifies over-represented GO terms or pathways in a filtered list of genes.

Enrichment analysis

Click the filtered Filtered feature list data node
Click Biological interpretation in the task menu
Click Gene set enrichment then select Gene set database to perform GO enrichment analysis (Figure 1)

Flow Documentation > Performing biological interpretation > Screenshot 2023-08-09 at 15.20.54.png

Select the latest gene set from geneontology.org from the Gene set database drop-down menu
Click Finish

A GO enrichment task node will be added to the pipeline (Figure 2).

Flow Documentation > Performing biological interpretation > Screenshot 2023-08-09 at 15.21.40.png

Double-click the Gene set enrichment task node to open the task report (Figure 3)

Flow Documentation > Performing biological interpretation > Screenshot 2023-08-09 at 15.23.05.png

The GO enrichment task report spreadsheet lists GO terms by ascending p-value with the most significant GO term at the top of the list. Also included are the enrichment score, the number of genes from that GO term in the list, and the number of genes from that GO term that are not in the list.

To view the genes associated with each GO term, select Flow Documentation > Performing biological interpretation > image2017-9-1 14:47:12.png to open the extra details page. To view additional information about a GO term, click the blue gene set ID to open the linked geneontology.org entry in a new tab.

For more information about GO enrichment analysis, please see the Gene Set Enrichment user guide.

KEGG enrichment analysis

KEGG enrichment analysis identifies pathways that are over-represented in a gene list data node.

Click the filtered Filtered feature list data node
Click Biological interpretation in the task menu
Click Gene set enrichment then select KEGG database
Click Finish in the configuration dialog to run KEGG analysis with the Homo sapiens KEGG database

A Pathway enrichment task node will be added to the pipeline (Figure 4).

Flow Documentation > Performing biological interpretation > Screenshot 2023-08-09 at 15.24.02.png

Double-click the Pathway enrichment task node to open the task report

The Pathway enrichment task report is similar to the Enrichment analysis task report (Figure 5).

Flow Documentation > Performing biological interpretation > Screenshot 2023-08-09 at 15.24.17.png

To view an interactive KEGG pathway map, click the pathway ID (Gene set column).