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During a previous section of this tutorial, a spreadsheet named unexplained_regions was generated. This spreadsheet contains locations where reads map to the genome but are not annotated by the transcript database, in this case, RefSeqGene. Theunexplained_regions spreadsheet is potentially very interesting as it may contain novel findings.

Right click column 6. Average Coverage and select Sort Descending from the menu
Select Find Overlapping Genes from the Tools option in the command toolbar (Figure 1)

Genomics Suite Documentation > Analyzing the unexplained regions spreadsheet > fig45.png

Select Add a new column with the gene nearest to the region in the Find Overlapping Genes dialog (Figure 2)
Select OK

Genomics Suite Documentation > Analyzing the unexplained regions spreadsheet > fig46.png

Select RefSeqTranscripts – 2014-01-03 from the Output Overlapping Features dialog (Figure 3)

Please note that it is recommended that you annotate with the same database as when you performed mRNA quantification.

Select OK

Genomics Suite Documentation > Analyzing the unexplained regions spreadsheet > fig47.png