We will be using the RNA-seq workflow to analyze RNA-seq data throughout this tutorial. The commands included in the RNA-seq workflow are also available form the command toolbar, but may be labeled differently.
- Select the RNA-seq workflow by selecting it from the Workflow drop-down menu in the upper right-hand corner of the Partek Genomics Suite window (Figure 1)
The Partek Genomics Suite software can import next generation sequencing data that has been aligned to a reference genome. Two standard types of alignment formats can be imported: .BAM and .SAM. It is also possible to concert ELAND .txt files to .BAM files with the converter found in the Tools menu in the main command bar. The data used in this tutorial was aligned using the Partek Flow software and saved as .BAM files.- To import the .BAM files, select Import and Manage Samples from the Import section of the RNA-seq workflow. The Sequence Import dialog box will open (Figure 2)
