One of the main functions of GO enrichment is to find the overrepresentation of functional categories in a gene list. With the Gene_List.txt spreadsheet selected:

• From the Gene Expression workflow, choose Biological Interpretation followed by Gene Set Analysis
• Select the GO Enrichment radio button in the Gene Set Analysis dialog (Figure 1) followed by Next
• Make sure the Gene_List.txt spreadsheet is chosen from the dropdown list and click Next

You have the choice to use the Fisher's Exact or Chi-Square test. Both are acceptable and you can always test both by re-running the analysis. You can also restrict the analysis to functional groups with more than or fewer than a specified number of genes. Restricting the analysis to GO groups with fewer than 150-200 genes will increase the speed of analysis and exclude large groups which may not be too informative. If analysis time is not a concern, you can just use the default settings.

• Select the Use Fisher's Exact test radio button (Figure 2)
• Make sure the Invoke gene ontology browser on the result checkbox is selected
• Leave all other settings as default and click Next
  

Partek Genomics Suite supports different types of mapping files. These are library files that define how genes are organized into functional groups. For an explanation of each type of mapping file, click on the help icon ().

Your Rating:

Thanks for voting!

Please Wait

Results:

0

rates