Partek Flow Documentation

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When Other is selected, a specific value of the effective genome size needs to be specified in bps as unit (Figure 2).

 

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SubtitleTextSpecify other species effective genome size by manually type in the value
AnchorNameg size other

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When sample attribute is not specified, for instance there are only two sample -- ChIP and mock as sample name, the peak detection pairs needs to be manually defined (Figure 1).

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When select MACS2 task, the default option is to use sample attribute to add multiple pairs at one button click (Figure 4)

 

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SubtitleTextSpecify IP vs control pairs based on sample attributes
AnchorNamechip_attribute

 

There are IP-Input pair in each time point, so the pair attribute is Time; Control attribute is the attribute contains IP and input group, which is ChIP, the control term is labeled as Input in the example, when click Generate pairs, the two pairs will be automatically added to the Pairs table at once (Figure 5).

 

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SubtitleTextTwo IP vs input sample pairs are added in the Pairs table
AnchorNamechip_pair_table

If multiple pairs are added in the Pairs table, the peak detection is performed on each pair independently.

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In the task report, each pair will generate a list of peaks displayed in a table (Figure 6)

 

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SubtitleTextPeaks report on each IP vs control pair
AnchorNamechip_report

In the report table, each row is a peak, besides genomic location of the peak region, it also include the following information:

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